16 AUG 2019 · As we know, sharks have been in existence for over 500 million years. From an evolutionary perspective of vies, they are among the oldest animals with a “modern” immune system not unlike that of humans. Shark blood contains large quantities of urea that makes their antibodies much more robust than that of other vertebrates. While urea protects sharks from dehydration, it can also destabilize sensitive protein molecules such as antibodies. https://www.creative-biolabs.com/blog/index.php/native-shark-antibody-discovery-used-in-shark-monoclonal-antibody-production/
16 AUG 2019 · The unique guinea pig hybridoma technology is adopted to generate monoclonal guinea pig antibodies. Through fusing with our specific myeloma as partner, guinea pig hybridoma can be developed with excellent stability and productivity. https://www.creative-biolabs.com/blog/index.php/creative-biolabs-makes-breakthrough-in-guinea-pig-monoclonal-antibody-production-with-hybridoma-technology/.
16 AUG 2019 · Guinea pig, which is suggested as domesticated descendants of a closely related species of cavy, is a kind of rodent that does not exist naturally in the wild. It is one of the earliest species that has been applied in the field of research and investigation for more than 20 decades. However, as the genetic research of guinea pig (such as guinea pig antibody) has fallen behind during the early 20th century, it becomes less popular than mice and rats in a variety of research (such as humanized mice antibody). Even though, it is still an ideal source to generate antibodies for extreme allergic reactions or anaphylaxis research. It is also treated as an important model for the study of various infectious diseases, such as tuberculosis and diphtheria. https://www.creative-biolabs.com/blog/index.php/creative-biolabs-makes-breakthrough-in-guinea-pig-monoclonal-antibody-production-with-hybridoma-technology/
16 AUG 2019 · The first single domain antibody engineered from the heavy-chain antibodies and found in camelids has greatly stimulated the development of antibody market. Heavy-chain antibodies found in camelids can overcome or relieve immune system diseases. https://www.creative-biolabs.com/blog/index.php/single-domain-antibody-made-it-possible-for-curing-diseases-with-antibody-drugs/
16 AUG 2019 · The Rapid Development of Antibody Drugs
Except brain, the most sophisticated part in a human body is the immune system. In a sense, we are living in a world surrounded by various bacteria. They never cease disturbing us by taking over our living surroundings as their ideal place for breeding. Under this circumstance, antibody is the only one that can take the responsibility of an inner protector. Each antibody model has its own mission. When foreign objects, including microorganism, allergen and toxin, are trying to invade the immune system, antibodies will send out unique chemical signals on receiving which our immune system starts working. https://www.creative-biolabs.com/blog/index.php/single-domain-antibody-made-it-possible-for-curing-diseases-with-antibody-drugs/
16 AUG 2019 · Reagents and apparatus of Protein Expression, Isolation and Purification
Reagents
LB fluid medium: Tryptone 10g, yeast extract 5g, NaCl 10g.Mix all together with distilled water to 1000mL.
Ampicillin: 100mg / mL
Sample: Buffer Solution. 100 mM NaH2PO4, 10 mMTris, 8M Urea, 10 mM2-ME, pH8.0
Washing Buffer: 100 mM NaH2PO4, 10 mM Tris, 8 M Urea, pH6.3
Elution Buffer: 100mM NaH2PO4, 10 mMTris, 8M Urea, 500 mM Imidazole, pH8.0
IPTG https://www.creative-biolabs.com/blog/index.php/protein-expression-isolation-and-purification/
16 AUG 2019 · Experiment Principles of Protein Expression, Isolation and Purification
Expression of gene clone in cells is of great significance in both theoretical research and experimental applications. We can explore and study gene function and expression mechanism expression with gene expression. What’s more, the encoded protein by cloned gene expression can be used in researching the structure and function.
E.coli (Escherichia coli ), whose exogenous expressing productivity is much higher than that of other gene expression systems, is currently the most widely used protein expression system. The number of target protein expressed may even exceed 80% of the total protein in bacteria. In this experiment, the plasmid carrying target protein overexpresses recombinant chloramphenicol acyltransferase protein with 6 consecutive histidine residues in E. coli BL21 under the induction of IPTG in 37 ℃. The protein can be purified with chromatographic media formed by immobilizing nickel ions (Ni2 +) with covalent coupling nitrilotriacetic acid (NTA), which is in fact Metal Chelate Affinity Chromatography (MCAC). The purity of the protein can be analyzed by polyacrylamide gel electrophoresis. https://www.creative-biolabs.com/blog/index.php/protein-expression-isolation-and-purification/
16 AUG 2019 · Protein Expression, Isolation and Purification
Learn the approaches and meaning of the expression of cloned genes.
Learn the method of recombinant protein affinity chromatography, isolation and purification
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